Unchecked 'Display other X/Y parameters than in parent plot' as default in Gates Preferences.Updated EULA to raise the awareness of the fact that you may not install FlowJo on a VM.Better interface for Licensing Preferences Panel in the case where a computer has many hardware addresses.Added a field to allow specification of R's path in prefs (Diagnostic Panel).Increased the number of parameters that FlowJo can analyze to 1000.You'll find these as 'plugins' in the populations band - each offers one-click access to powerful tools to reduce any number of parameters to two and to query a database to name unknown populations. FlowJo 10.2 includes a set of tools for discovery including downsampling, tSNE and CellOntology. The open plugin architecture of 10.2 allows access to cutting edge tools that can be integrated into your current analysis workflows, and we will be releasing developer documentation so any tool can be integrated into FlowJo. Be sure your version of FlowJo Enterprise is new enough before installing this version of FlowJo.įlowJo can now use plugins that can perform a variety of functions, including the creation of populations, creation of derived parameters, and actions when opening or saving a workspace. Note: This version of FlowJo is compatible with FlowJo Enterprise v2.0 or the newly released FlowJo Enterprise 2.1. Improved interactions with BD® Research Cloud: Save and load options throughout the software now include the BD ® Research Cloud as an option.Native Cluster Explorer: Display line charts, bar charts, heat maps, and dimensionality reduction plots of clustered data in FlowJo without the use of a plugin.Clone gates: Gates with the same name can now be linked together as ‘clones’ so that their position is synchronized.Auto-Quad gates: Automatically create quads and rectangle gates based on range gates, bi-sector gates or the data itself.Spectral similarity scores: Calculate the cosine index for acquired data to evaluate reagent choices made in panel design based on data from your specific instrument and experimental conditions.MEM – Marker Enrichment Modeling (MEM): Re-name clusters automatically and quantitatively based on their marker expression with a human- and machine-readable label.Autofluorescence extraction in traditional (gate-based) compensation: Use an unstained cell data file and true zero as the background value to model autofluorescence for extraction.Embed: Forward propagate clustering or dimensionality reduction results to new files with the same parameters.If you would like the uncompensated always overlaid by default, check this box in the preferences. Overlay uncompensated – The matrix editor will allow you to overlay your uncompensated data on top of the compensated to view the difference. If you prefer to not see this confirmation box, uncheck the box here in the preferences. These preferences can be very useful since you have the control to set the default values of whatever keyword you wish to automatically assign compensation single stain controls to the compensation group upon loading the files! Matrix EditorĬonfirm Edit – when you click the “Edit” button in the matrix editor, a confirmation box will open asking you to confirm that you wish to edit the matrix. Some other common keywords are “TUBENAME” and “SampleID”. If you prefer to use a different keyword, you can remove the default $FIL strings using the X icon next to them in this interface and then click the “Add” button to add whatever keywords you would like to use. Thus, any FCS files put into a workspace that contains the text “comp” or “unstained” in the $FIL, or filename, will be included in the compensation group in your workspace when loaded. The default values set are “comp” and “unstained”. $FIL – is the FCS file keyword for the name of the data file written by the cytometer. The “Group Inclusion” can be used to make files with certain file names get automatically included in the group when they are loaded into FlowJo. Select the Compensation button in the Preferences tool to open the Compensation preferences. Set your default preferences for compensation
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